General Cell Based Assays
Eurofins Discoverx PathHunter eXpress rCHRM4 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0870E2RCP2M)
The PathHunter rCHRM4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. acetylcholine) based activation of rCHRM4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged rCHRM4 and EA-tagged β-Arrestin-2. Activation of rCHRM4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IL21R/IL2RG Dimerization Assay, 2pl Kit. (93-1035E3CP0M)
The PathHunter IL21R/IL2RG Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-21) induced dimerization of IL21R/IL2RG receptors. This product uses EFC technology. The cell line included overexpresses IL21RG target protein fused to PK alongside IL2R target protein fused to EA. Upon ligand-induced dimerization of IL21R/IL2RG, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress cINSRa Functional Assay, 10-Plate Kit. (93-1016E3CP16L)
The PathHunter eXpress cINSRa Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated cINSRa dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged cINSRa and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Cynomolgus monkey version of the protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human HTR2C (5-HT2C) β-Arrestin Cell-Based Assay Kit (U2OS)
The PathHunter Human HTR2C(VSV) (5-HT2C) β-Arrestin Stable Cell Line Assay (U2OS) contains a stable clonal cell line used to measure HTR2C(VSV) (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human GPR120S β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human GPR120S β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GPR120S (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
ABclonal Technology TrKC Rabbit pAb
This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. Mutations in this gene have been associated with medulloblastomas, secretory breast carcinomas and other cancers. Several transcript variants encoding different isoforms have been found for this gene.
TRIBIOSCIENCE INC Mitochondrial Complex III Activity Colorimetric Assay 100 Tests
The activity of Complex III is directly measured through the increase of reduction of cytochrome c at absorbance of 550nm. This kit uses cytochrome c as substrate. The reduced cytochrome c is determined by recording the change in absorbance (A550). Applications Complex III Activity.
Bioassay Systems EnzyChrom™ Adipolysis Assay Kit. For quantitative assay of adipolysis and evaluation of drug effects on adipolysis.
For quantitative assay of adipolysis and evaluation of drug effects on adipolysis. Key Features: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 0.92 to 100 µg/mL (10 to 1000 µM) glycerol for colorimetric assays and 0.2 to 5 µg/mL for fluorimetric assays. Rapid and convenient.The procedure involves addition of a single working reagent and incubation for 20 min at room temperature. Robust and amenable to HTS assays. Potential interference by testing drugs is greatly reduced at 570nm. Compatible with culture media containing phenol red. Assays can be performed in 96 or 384-well plates. Method: OD570nm, or FL530/585nm. Samples: Cell culture media. Species: All. Procedure: Assay takes 20 min. Kit size: 200 tests. Detection limit: 0.92 µg/mL.
Cayman Chemical Glycogen FluorometrIc Detecto
A GnRH receptor antagonist (Kis 6 2.2 and 3.8 nM for the human mouse and rat receptors respectively) selective for the GnRH receptor over a panel of 43 receptors ion channels and enzymes at 1 UM prevents GnRH-induced increases in inositol phosphate production in HEK293 cells expressing the human GnRH receptor (KB 25 nM) oral or intravenous administration decreases plasma LH levels in castrated rats inhibits GnRH-A-induced increases in plasma LH and testosterone levels in non-castrated rats at 20 mg/kg
Cayman Chemical cGAS Stop SolutIon 1mL
cGAS Stop Solution has been tested and formulated to work exclusively with Cayman’s cGAS Inhibitor Screening Assay Kit (Item No. 701930).
Eurofins Discoverx PathHunter AGTRL1 Bioassay Kit, 10-Plate. (93-0250Y2-00122)
The PathHunter AGTRL1 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. apelin-13) based activation of AGTRL1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged AGTRL1 and EA-tagged β-Arrestin-2. Activation of AGTRL1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
ABclonal Technology Kallikrein 8 Rabbit mAb
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in tandem in a gene cluster on chromosome 19. The encoded protein may be involved in proteolytic cascade in the skin and may serve as a biomarker for ovarian cancer. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms.
Eurofins Discoverx PathHunter eXpress rDRD1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0620E2CP2L)
The PathHunter rDRD eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dopamine) based activation of rDRD GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged rDRD and EA-tagged β-Arrestin-2. Activation of rDRD stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Bioassay Systems EnzyFluo™ Bile Acid Assay Kit. For quantitative determination of total bile acids and evaluation of drug effects on bile acid metabolism.
For quantitative determination of total bile acids and evaluation of drug effects on bile acid metabolism. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. Linear detection range of 1 - 150 µM bile acids. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL530/585nm. Samples: Serum, plasma, urine and other biological samples. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 1 µM.